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Methods used to detect cholesterol’s condensing effect on phospholipids. Schematic showing (A) the area occupied per acyl chain in (i) egg PC and (ii) egg PC/cholesterol (1/1, mol/mol) as a function of surface pressure and (B) a plot of the mean area per molecule as a function of the mole fraction of phospholipid present in a mixture of cholesterol (C) and phospholipid (P) at constant surface pressure.
