Table 1.

Comparison of MRD testing with next-generation flow cytometry and next-generation sequencing

Characteristic	Next-generation flow cytometry	Next-generation sequencing
Requires baseline sample	No	Yes, in order to identify clone to track
Sensitivity	<10−5	<10−6
Sample requirements	>5 million cells (more cells to increase sensitivity to 10−6)	<2 million cells
Sample processing	Requires fresh sample	For Clonoseq tracking: fresh or frozen sample
Assessment for hemodilution	Yes	Not possible
Turnaround time	2-4 days	7 days
Commercial availability	Reference or experienced in-house laboratory	Clonoseq (Adaptive); LymphoTrack for in-house use

Table adapted from Kumar et al.⁹