FIG 1.

Detection of CDV and CPV Abs in serum from eastern coyotes and red and gray foxes. (A) Serum samples were screened for CDV- and CPV-specific Abs as detailed in the text. Pictures of representative ELISA plate wells are shown with animal identification numbers above each image. Visual scoring results (+ or −) are provided below each image. (B) To validate the visual scoring, the absorbance values for representative samples were determined. The positive threshold (red line) was determined based on the absorbance value of the TiterCHEK kit (Zoetis) positive control. (C) Regional differences in seroprevalence. The map divides the Commonwealth of Pennsylvania into six sectors (NW, northwest; NC, north central; NE, northeast; SW, southwest; SC, south central; SE, southeast). Each sector is color coded as indicated in the figure to reflect the number of Ab-positive animals for CDV or CPV. Gray coloration indicates insufficient data to determine percentages. Data for red and gray foxes were combined. ND, not determined.