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TM and OMV fractions were prepared using early stationary-phase liquid-grown cultures. The TM and OMV preparations were resuspended in PBS, and the protein concentration was determined: 10 μg protein per sample was loaded onto 12% SDS-PAGE gels and analyzed by (A) Coomassie blue staining or (B) Western blot, using anti-RNA polymerase antisera and REVERT total protein stain as the loading control. Lack of IPC does not cause cell lysis or impact the differential TM/OMV protein profile.
