TABLE 1.
PCR results for EBV and β-globin with whole-blood, serum, and plasma samples from healthy donors and patients with EBV-related disease
| Population | Qualitative EBNA-1 PCR (no. of positive samples/no. of samples tested)
|
Quantitative EBNA-1 PCR (no. of samples with values above cutoff value/no. of samples tested)a
|
No. of serum or plasma samples/no. of samples tested additionally positive by BamHI-W-repeat PCR | β-Globin PCR result (no. of positive samples/no. of samples tested)
|
Range of EBV DNA load (no. of copies/ml)
|
||||
|---|---|---|---|---|---|---|---|---|---|
| Whole blood | Serum or plasma | Whole blood | Serum or plasma | Whole blood | Serum or plasma | Whole blood | Serum or plasma | ||
| Healthy EBV carriers (n = 11) | 1/18 | 1/18 | 0/1 | 0/1 | 0/18 | 18/18 | 13/18 | ||
| Burkitt's lymphoma patients (n = 12) | 12/12 | 12/12 | 12/12 | 8/12 | 12/12 | 12/12 | 14,600–4,591,900 | 4,400–108,600 | |
| African controls (n = 12) | 12/12 | 7/12 | 3/12 | 0/12 | 12/12 | 12/12 | 3,800–17,500 | ||
| PTLD patients (n = 4) | 35/35 | 15/35 | 30/35 | 0/15 | 2/35 | 35/35 | 35/35 | 2,600–308,000 | |
| HIV infected patients (n = 2) | 2/2 | 0/9 | 2/2 | 0/9 | 0/9 | 2/2 | 0/9 | 15,600–89,400 | |
| Infectious mononucleosis patients (n = 5) | 4/5 | 0/5 | 4/5 | 0/5 | 4/5 | 5/5 | 4/5 | 34,000–600,000 | |
a
Quantitative EBNA-1 PCR cut off value, 2,000 EBV DNA copies/ml of blood, serum, or plasma.