Figure 4.

Progerin expressing ECs exert paracrine senescence. (A) Schematic representation depicting the separation of EC- from non-EC populations in lung tissue. (B) qPCR analysis of senescence and SASP marker genes in non-EC populations of WT vs Prog-Tg mice (>25 weeks). (C) Schematic representation of the in vitro co-culture setup. (D) qPCR analysis of senescence and SASP marker genes in WT fibroblasts co-cultured in the presence of WT or Prog-Tg ECs (n=4-9). Paired two-tailed Students t test was applied for in vivo experiments with littermate pairs and unpaired two-tailed Students t test for in vitro co-culture experiments. ns=non-significant, *p<0.05, **p<0.01, ***p<0.001.