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Effects of different intensities of mechanical stress on YAP. (A) The YAP localization of VSMCs was detected by immunocytochemistry. (B, C) Quantification of nuclear YAP amount in VSMCs. (D) The levels of phosphorylated YAP and cyclin D1 (ccnd1) in VSMCs were detected by western blotting. Values are expressed as means±SD. **P < 0.01, compared with 0% elongation group.
