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Replication of ZIKV envelope and NS2A variants is attenuated in A549 cells. Multistep growth curves of WT ZIKV and variants in A549 (A), Vero (B), and C6/36 cells (C). Each cell line was infected with each virus at an MOI of 0.1, and virus titers in the supernatant were quantified by focus-forming assay. Dotted lines indicate the limit of detection. Data are from 3 independent experiments. **, P < 0.01 (two-way ANOVA).
