FIG 10.
Interactions between FcγRIIA and monomeric and multimeric VRC01 are differentially affected by complement. (A and B) Interactions of monomeric and multimeric VRC01/HIV-1iGFP/JRFL immune complexes with FcγRIIA were measured in the absence (A) and presence (B) of guinea pig complement. Virus capture was evaluated by subsequent p24 ELISA. All VRC01 variants and nonspecific controls were tested at 2 μg/mL. Experiments were performed in triplicate and repeated three times. Lines represent median OD450.values. P values were calculated using Kruskal-Wallis with Dunn’s multiple-comparisons tests.