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Coomassie staining of purified VRC01 variants. (A and B) All VRC01 variants were examined with SDS-PAGE under nonreducing (A) and reducing (B) conditions. The percentage of acrylamide in the nonreducing gel (7.5%) differs from that in the reducing gel (10%). A total amount of 3 μg of each antibody was loaded per well. Lane MW contains the All Blue Prestained Protein Standard (Bio-Rad). Heavy-chain (HC) and light-chain (LC) bands are indicated.
