FIG 7.
VRC01 multimerization augments HIV-1 internalization in the presence of complement. (A to D) VRC01RGY- and VRC01-mediated uptake was measured in the absence of complement with monocytes (A) or neutrophils (B) and in the presence of complement with monocytes (C) or neutrophils (D). (E and F) VRC01RGY-mediated significantly greater HIV-1iGFP-JRFL internalization in the presence of complement compared to VRC01 in both monocytes (E) and neutrophils (F). Results are reported as virus internalization score (percent positive cells × MFI) or fold change compared to the DEN3 control. Experiments were performed in triplicate and were repeated with cells from the blood of six healthy donors. Solid lines indicate median internalization score or fold change in viral uptake. P values were calculated using Kruskal-Wallis with Dunn’s multiple-comparisons tests (panels A to D) or using Mann-Whitney tests (panels E and F).