TABLE 1.

Details of primers and probe system used in PCR-based assays for H. pylori detection and mutation identification

Primer or probe	Target (reference)	Primer or probe sequence (5′–3′)
Conventional PCR
VAC3624F	vacA	GAG CGA GCT ATG GTT ATG AC
VAC3853R	vacA	ACT CCA GCA TTC ATA TAG A
Hp1	16S rRNA (16)	CTG GAG AGA CTA AGC CCT CC
Hp2	16S rRNA (16)	ATT ACT GAC GCT GAT TGT GC
LightCycler PCR
23F	23S rRNA (14)	CAA CCA GAG ATT CAG TGA AA
23R	23S rRNA (14)	GTG CTA AGT TGT AGT AAA GGT
23Pr	23S rRNA (14)	Cy5-GGC AAG ACG GAA AGA CCC-biotin