TABLE 3.
Performance of PCR-based probe melting point hybridization assay to determine clarithromycin susceptibility of H. pylori directly from 142 gastric biopsy samples compared with that of the culture-based disk diffusion test
| Result of 23S rRNA mutation assay | No. of samples having indicated:
|
|||
|---|---|---|---|---|
| Biopsy culture status
|
Clarithromycin susceptibilitya
|
|||
| + (n = 56) | − (n = 86) | Sensitive | Resistant | |
| Wild type | 48 | 1 | 47 | 1b |
| A→G | 3 | 0 | 1 | 1c |
| A→C | 1 | 0 | 0 | 1 |
| PCR negative | 4 | 85 | 3 | 1 |
a
Determined by disk diffusion test on matched cultures (n = 55) from positive biopsy samples.
b
Mixed population of clarithromycin-sensitive and -resistant variants and strains.
c
One matched culture not available.