TABLE 1.
Oligonucleotides used in this study
| Name | Direction and type | Sequence (5′→3′)a |
|---|---|---|
| rcDNA oligonucleotide bait | Biotin-TEG-ACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGC | |
| HBV total DNA | Forward primer | CCGTCTGTGCCTTCTCATCTG |
| Reverse primer | AGTCCAAGAGTYCTCTTATGYAAGACCTT | |
| Probe | FAM-CCGTGTGCACTTCGCTTCACCTCTGC-TAMRA | |
| HBV cccDNA | Forward primer | ATGGAGACCACCGTGAACGCCC |
| Reverse primer | TCCCGATACAGAGCTGAGGCGG | |
| Probe | FAM-TTCAAGCCTCCAAGCTGTGCCTTGGGTGGC-TAMRA | |
| Mitochondrial DNA | Forward primer | CCCTCTCGGCCCTCCTAATAACCT |
| Reverse primer | GCCTTCTCGTATAACATCGCGTCA | |
| DDB2 crRNAb | Sense oligonucleotide | CACCGGGAGGGCTACATCCTTGGCG |
| Antisense oligonucleotide | AAACCGCCAAGGATGTAGCCCTCCC | |
| DDB2 indel PCR | Forward primer | TCATTTCTCTCTGTGGCAGGGG |
| Reverse primer | GAACTGCTCACCCCTTTGATGA |
a
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.
b
The sticky ends for cloning are in italics and underlined.