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. 2022 Jan 7;11:777627. doi: 10.3389/fcimb.2021.777627

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Copyright © 2022 Wang, Liu, Li, Li, Xu, Niu and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Longitudinal dynamics of gingival crevicular fluid (GCF) microbiota. (A) Overview of the study design and sample collection. (B) Venn diagram of amplicon sequence variants (ASVs) in six comparison groups. Day 0: before treatment; day 7: 7 days after treatment; day 14: 14 days after treatment; day 30: 30 days after treatment; day 90: 90 days after treatment; day 180: 180 days after treatment. (C) The alpha diversity of the GCF microbiota over time. The Simpson index compared between microbiota of each time point and before treatment. Significance was measured using the Wilcoxon rank-sum test (P > 0.05, ns).