TABLE 2.
PCR-RFLP and partial sequencing analyses of the gltA gene for Bartonella species identification in 18 infected ticks
| Origin | Tick | PCR-RFLP profilea | Closest Bartonella species (% DNA similarity based on 273 bp of the gltA gene) |
|---|---|---|---|
| Red Fern Ranch | 4 | B. quintana-like | B. quintana (97.8) |
| 5 | B. quintana-like | B. quintana (99.6) | |
| 12 | B. quintana-like | B. quintana (100.0) | |
| Windy Hill Open Space Reserve | 15 | B. henselae-like | B. henselae (99.6) |
| 17 | B. henselae-like | B. henselae (98.5) | |
| 22 | B. henselae-like | B. henselae (99.3) | |
| 34 | B. henselae-like | B. henselae (97.4) | |
| 63 | Bartonella strain cattle-1-like | Bartonella strain cattle-1 (99.6) | |
| 70 | Mixed type of B. henselae and B. vinsonii subsp. berkhoffii | B. vinsonii subsp. berkhoffii (98.9) | |
| 73 | Bartonella strain cattle-1-like | Bartonella strain cattle-1 (99.3) | |
| 74 | B. henselae-like | B. henselae (98.9) | |
| 75 | Mixed type of B. henselae and Bartonella cattle-1 strain | Bartonella strain cattle-1 (99.6) | |
| 80 | B. henselae-like | B. henselae (100.0) | |
| 81 | Bartonella strain cattle-1-like | Bartonella strain cattle-1 (99.6) | |
| 93 | Bartonella strain cattle-1-like | Bartonella strain cattle-1 (99.3) | |
| 143 | Unrecognized type | B. washoensis (99.3) | |
| 146 | Unrecognized type | B. washoensis (100.0) | |
| 147 | Unrecognized type | B. washoensis (100.0) |
PCR-RFLP profile of B. quintana: 310-bp band with TaqI digestion, 400-bp band with HhaI digestion, and 150- and 200-bp bands after MseI digestion. PCR-RFLP profile of B. henselae: 90-, 150-, and 190-bp bands with TaqI digestion, 190- and 220-bp bands with HhaI digestion, and 150- and 200-bp bands with MseI digestion. PCR-RFLP profile of Bartonella strain cattle-1: 150-and 250-bp bands with TaqI digestion, 400-bp band with HhaI digestion, and 140- and 200-bp bands with MseI digestion. PCR-RFLP profile of the mixed type of B. henselae and B. vinsonii subsp. berkhoffii: 90-, 150-, and 190-bp bands with TaqI digestion, 190-, 220-, and 400-bp bands with HhaI digestion, and 80-, 150-, 200-, and 210-bp bands with MseI digestion. PCR-RFLP profile of the mixed type of B. henselae and Bartonella strain cattle-1: 90-, 150-, 190-, and 250-bp bands with TaqI digestion, 190-, 220-, and 400-bp bands with HhaI digestion, and 140- and 200-bp bands with MseI digestion.