Fig. 4.

miR-26a regulates pro-inflammatory cytokines via PGE2 production. BMDMs were pre-treated with miR-26a mimic (50 nM) (A) or miR-26a inhibitors (100 nM) (B) for 24 h and incubated with LPS for the specified time. PGE2 concentrations in culture supernatants were measured by ELISA. C BMDMs were transfected with control or miR-26a mimic together with or without PGE2 addition for 24 h followed by incubated with LPS for 24 h. TNF-α, IL-6, and IL-1β concentrations in culture supernatants were measured by ELISA respectively. Data shown in are the mean ± SD. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001. Data are representative of three independent experiments with similar results. Values in A–B were compared using two-way ANOVA with Bonferroni’s test, and values in C were compared using one-way ANOVA with Bonferroni’s test.