| Strain, strain background (Escherichia coli) |
BL21(DE3) Competent E. coli
|
New England BioLabs |
Cat#C2527I |
Chemically competent E. coli. |
| Strain, strain background (Escherichia coli) |
MAX Efficiency DH10Bac Competent Cells |
Thermo Fisher |
Cat#10361012 |
Chemically competent E. coli. |
| Cell line (Mus musculus) |
Ptk2+/+, Trp53-/- MEFs; WT MEFs |
ATCC |
CRL-2645; RRID: CVCL_8955
|
|
| Cell line (Mus musculus) |
Ptk2-/-, Trp53-/- MEFs; FAK KO MEFs |
ATCC |
CRL-2644; RRID:CVCL_8954
|
|
| Cell line (Mus musculus) |
Bcar1-/- MEFs + Control Vector; Cas KO control MEFs |
Meenderink et al., 2010
|
N/A |
|
| Cell line (Mus musculus) |
Bcar1-/- MEFs + CasWT Vector; Cas KO CasWT MEFs |
Meenderink et al., 2010
|
N/A |
|
| cell line (Mus musculus) |
Bcar1-/- MEFs + Cas15 F Vector; Cas KO CasY15F MEFs |
Meenderink et al., 2010
|
N/A |
|
| Cell line (Spodoptera frugiperda) |
Sf9 Cells |
Gibco |
Cat#11496015 |
|
| Antibody |
Mouse monoclonal anti-Paxillin (Clone 349/Paxillin) |
BD Biosciences |
Cat#610051; RRID: AB_397463
|
(1:100) |
| Antibody |
Mouse monoclonal anti-FAK (Clone 4.47) |
Millipore |
Cat#05–537; RRID: AB_2173817
|
(1:1000) |
| antibody |
Rabbit monoclonal anti-p130 Cas (E1L9H) |
Cell Signaling Technology |
Cat#13846; RRID: AB_2798328
|
(1:1,000) |
| Antibody |
Rabbit polyclonal anti-beta Actin |
Abcam |
Cat#ab8227; RRID: AB_2305186
|
(1:5000) |
| Antibody |
Goat polyclonal anti-Mouse IgG, Alexa Fluor 568 |
Thermo Fisher |
Cat#A-11004; RRID: AB_2534072
|
(1:250) |
| Antibody |
Goat polyclonal anti-Mouse IgG, HRP |
Santa Cruz Biotechnology |
Cat#sc-2005; RRID: AB_631736
|
(1:10,000) |
| Antibody |
Goat polyclonal anti-Rabbit IgG, HRP |
Santa Cruz Biotechnology |
Cat# sc-2030; RRID: AB_631747
|
(1:10,000) |
| Sequence-based reagent |
ON-TARGETplus Mouse Bcar1 (12927) siRNA - SMARTpool |
Horizon Discovery (Dharmacon) |
Cat#L-041961-00-0005 |
Commercially available |
| Sequence-based reagent |
ON-TARGETplus Non-targeting Control Pool |
Horizon Discovery (Dharmacon) |
Cat#D-001810-10-05 |
Commercially available |
| Sequence-based reagent |
ON-TARGETplus Mouse Ptk2 siRNA - SMARTpool |
Horizon Discovery (Dharmacon) |
Cat#L-041099-00-0005 |
Commercially available |
| Chemical compound, drug |
Alexa Fluor 647 C2 Maleimide |
Invitrogen |
Cat#A20347 |
|
| Chemical compound, drug |
Alexa Fluor 488 C5 Maleimide |
Invitrogen |
Cat#A10254 |
|
| Chemical compound, drug |
Alexa Fluor 568 C5 Maleimide |
Invitrogen |
Cat#A20341 |
|
| Chemical compound, drug |
SNAP-Surface Alexa Fluor 546 |
New England BioLabs |
Cat#S9132S |
|
| Chemical compound, drug |
SNAP-Surface Alexa Fluor 488 |
New England BioLabs |
Cat#S9129S |
|
| Chemical compound, drug |
Intracellular pH Calibration Buffer Kit (Nigericin/Valinomycin) |
Invitrogen |
Cat#P35379
|
|
| Software, algorithm |
MATLAB |
Mathworks |
|
|
| Software, algorithm |
FIJI |
https://fiji.sc
|
RRID:SCR_002285
|
|
| Other |
POPC |
Avanti Polar Lipids |
Cat# 850457C |
|
| Other |
PEG5000-PE |
Avanti Polar Lipids |
Cat# 880230C |
|
| Other |
DGS-NTA-Ni |
Avanti Polar Lipids |
Cat# 790404C |
|
| Other |
NBD PC |
Avanti Polar Lipids |
Cat# 810130C |
|
