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. 2022 Jan 26;9(2):e1133. doi: 10.1212/NXI.0000000000001133

Figure 2. PGRN Attenuates EAU and Inhibits Th1 and Th17 Immune Response.

Figure 2

(A) Quantitative RT-PCR analysis of PGRN expression in the retinal tissues from EAU mice at different time points after immunization. (B) B10RIII mice were immunized for EAU and injected intraperitoneally with rPGRN or PBS every other day from days 7–13 postimmunization. Quantification of clinical score of 2 EAU groups from day 7 to day 13 after immunization was shown. (C) Quantification of intracellular expression of IL-17, IFN-γ, and CD25+Foxp3+ by CD4+ T cells (Th1, Th17, and Treg, respectively) in the spleen from the 2 groups. (D) Quantitative RT-PCR analysis of IFN-γ, IL-17, IL-10, and foxp3 in the splenocytes from PGRN- or PBS-treated mice after immunization on day 13. Data are shown as mean ± SEM from 2 to 3 independent experiments with total of 10 mice per group. *p < 0.05, **p < 0.01, ***p < 0.001, and NS, not significant. EAU = experimental autoimmune uveitis; IL = interleukin; IFN = interferon; PBS = phosphate-buffered saline; PGRN = progranulin; RT-PCR = real-time PCR; Th = T helper; Treg = regulatory T.