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. 2022 Jan 26;9(2):e1133. doi: 10.1212/NXI.0000000000001133

Figure 4. Recombinant PGRN Inhibits IRBP-Reactive Th1 and Th17 Cell Expansion and Promotes IRBP-Reactive Treg Cell Expansion.

Figure 4

(A and B) Spleen cells from EAU mice were stimulated with IRBP651–670 in the presence or absence of rPGRN for 72 hours and then assessed for intracellular expression of IFN-γ, IL-17, and Foxp3 by CD4+ T cells by flow cytometry (n = 8). (A) Representative flow cytometry dot plots. (B) Histograms of IFN-γ, IL-17, and Foxp3 by CD4+ T cells (Th1, Th17, and Treg, respectively). (C and D) Naive CD4+ T cells from spleen cells of normal C57BL/6 mice were stimulated with Th1, Th17, and Treg cell polarization conditions in the presence or absence PGRN for 72 hours and then assessed for intracellular expression of IFN-γ, IL-17, and Foxp3 by CD4+ T cells by flow cytometry (n = 8). (C) Representative flow cytometry dot plots of the 2 groups. (D) Histograms of Th1, Th17, and Treg of the 2 groups. Data are shown as mean ± SEM from 2 independent experiments. *p < 0.05, **p < 0.01, and NS, not significant. EAU = experimental autoimmune uveitis; IL = interleukin; IFN = interferon; PGRN = progranulin; RT-PCR = real-time PCR; Th = T helper; Treg = regulatory T.