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. 2022 Jan 26;9(2):e1133. doi: 10.1212/NXI.0000000000001133

Figure 7. PGRN Skews the Balance of Macrophage Polarization From M1 to M2.

Figure 7

(A and B) Mononuclear cells were harvested from the CNS or spleen from PGRN−/− EAE mice and control EAE mice on day 22; then, the cells were stained for CD11b, F4/80, MHC-II, CD86, CD40, and CD206 (6 mice per group). (A) Flow cytometric analysis of the frequency of CD11b+F4/80+ macrophages in the CNS and spleen, respectively. (B and C) Flow cytometric analysis of the expression of MHC-II, CD86, CD40, and CD206 on the CD11b+F4/80+ cells in the CNS and spleen, respectively. (D) Bone marrow cells were cultured with the M-CSF for 7 days and then stimulated with or without PGRN on M1 or M2 polarizing conditions for another 2 days; then, the cells were analyzed for the expression of iNOS and Arg-1 by real-time PCR (n = 6). *p < 0.05, **p < 0.01; NS = not significant. Arg-1 = arginase 1; EAE = experimental autoimmune encephalomyelitis; iNOS = inducible nitric oxide synthase; PGRN = progranulin.