Figure 6.

NEH inhibits the proliferation, migration, and invasion of GC cells through GPR30 signalling. (a–d) A Wound healing assay investigated the effects of NEH (50 μg/ml), G15 (2.5 μM), and G1 (50 nM) on the migration of BGC-823 (a, b) and HGC-27 (c, d) cells. (e–g) Transwell assay on the effects of NEH, G15, and G1 on the invasion of BGC-823 and HGC-27 cells. The representative photographs show 48 h after drug administration. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001, compared with the NEH-treated group. (h) Western blot analysis on the expression of CDK2, cyclin A2, cyclin D1, MMP2, and MMP9.