Figure 3.

Pericyte-induced stabilization of the endothelium requires NOTCH3. A: representative images of endothelial junctions in vascular networks formed with endothelial cells alone, in coculture with Scr pericytes, or in coculture with N3^KO pericytes. VE-cadherin in white, p120-catenin in green, and DAPI in blue. Scale bar 50 μm. B: i) Higher magnification inset of VE-cadherin staining. Scale bar 15 μm. ii) Quantification of junctional VE-cadherin intensity. iii) Quantification of junctional widths for VE-cadherin. C: i) higher magnification inset of p120-catenin staining. Scale bar 15 μm. ii) Quantification of junctional p120-catenin intensity. iii) Quantification of junctional widths for p120-catenin. For Biii and Ciii, outliers were removed with ROUT test with Q of 1%. D: quantification of the coefficient of diffusive permeability of 70 kDa dextran for hEMV seeded with endothelial cells (ECs) alone, ECs with Scr PC, or ECs with N3^KO PC. For Bii, Cii, and D, whiskers were calculated using Tukey’s method. For all, P values calculated using ordinary one-way ANOVA with multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.