Pericyte-induced stabilization of the endothelium requires NOTCH1. A: representative images of endothelial junctions in vascular networks formed with siCtrl of siN1 endothelial cells alone, or in coculture with pericytes. Overlay with VE-cadherin in gray, p120-catenin in green, and DAPI in blue. Scale bar 50 μm. B: i) higher magnification inset of VE-cadherin staining. Scale bar 15 μm. ii) Quantification of junctional VE-cadherin intensity. iii) Quantification of junctional widths for VE-cadherin. C: i) Higher magnification inset of p120-catenin staining. Scale bar 15 μm. ii) Quantification of junctional p120-catenin intensity. iii) Quantification of junctional widths for p120-catenin. For Biii and Ciii, outliers were removed with ROUT test with Q of 1%. For Bii and Cii, whiskers were calculated using Tukey’s method. For all, P values calculated using ordinary one-way ANOVA with multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.