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. 2021 Sep 20;321(5):E606–E620. doi: 10.1152/ajpendo.00187.2021

Table 3.

Analysis of circadian gene expression in the gastrocnemius of control and alcohol-treated mice

Muscle One-Way ANOVA Results
Cosinar Results
0–24 h
24–48 h
0–24 h
24–48 h
P Value
P Value
P Value
P Value
Gene Control EtOH Control EtOH Con vs. EtOH
Clock 0.12 0.13 0.0004 0.09 0.59 0.05
Bmal1 <0.0001 <0.0001 <0.0001 0.01 <0.0001 0.03
Per1 <0.0001 0.61 <0.0001 0.33 <0.0001 0.40
Per2 <0.0001 <0.0001 <0.0001 0.01 0.02 0.03
Per3 <0.0001 0.0001 0.0002 0.27 0.20 0.03
Cry1 0.0002 0.02 0.02 0.97 <0.0001 0.001
Cry2 0.002 0.14 0.004 0.30 <0.0001 0.01
Reverbα <0.0001 <0.0001 <0.0001 0.01 0.89 <0.0001
Rorα 0.004 0.61 0.23 0.12 <0.0001 <0.0001
Myod1 <0.0001 0.001 0.01 0.52 <0.0001 0.11
Dbp <0.0001 <0.0001 <0.0001 0.01 0.0001 0.12
Tef 0.001 0.10 0.03 0.21 <0.0001 0.003
Bhlhe40 0.001 0.06 0.05 0.14 0.001 0.93

Analysis of circadian gene expression in the gastrocnemius of control and alcohol-treated mice. One-way ANOVAs were used to evaluate changes in gene expression overtime representative of circadian rhythmicity. A sum-of-squares F test was used to determine whether a single cosinor model fit both control and EtOH-treated groups to further indicate differences in rhythmicity between the two groups. Values are P values from control and alcohol-treated female mice at the time points indicated, with bold values indicating significance.