Table 3.
Analysis of circadian gene expression in the gastrocnemius of control and alcohol-treated mice
Muscle | One-Way ANOVA Results |
Cosinar Results |
||||
---|---|---|---|---|---|---|
0–24 h |
24–48 h |
0–24 h |
24–48 h |
|||
P Value |
P Value |
P Value |
P Value |
|||
Gene | Control | EtOH | Control | EtOH | Con vs. EtOH | |
Clock | 0.12 | 0.13 | 0.0004 | 0.09 | 0.59 | 0.05 |
Bmal1 | <0.0001 | <0.0001 | <0.0001 | 0.01 | <0.0001 | 0.03 |
Per1 | <0.0001 | 0.61 | <0.0001 | 0.33 | <0.0001 | 0.40 |
Per2 | <0.0001 | <0.0001 | <0.0001 | 0.01 | 0.02 | 0.03 |
Per3 | <0.0001 | 0.0001 | 0.0002 | 0.27 | 0.20 | 0.03 |
Cry1 | 0.0002 | 0.02 | 0.02 | 0.97 | <0.0001 | 0.001 |
Cry2 | 0.002 | 0.14 | 0.004 | 0.30 | <0.0001 | 0.01 |
Reverbα | <0.0001 | <0.0001 | <0.0001 | 0.01 | 0.89 | <0.0001 |
Rorα | 0.004 | 0.61 | 0.23 | 0.12 | <0.0001 | <0.0001 |
Myod1 | <0.0001 | 0.001 | 0.01 | 0.52 | <0.0001 | 0.11 |
Dbp | <0.0001 | <0.0001 | <0.0001 | 0.01 | 0.0001 | 0.12 |
Tef | 0.001 | 0.10 | 0.03 | 0.21 | <0.0001 | 0.003 |
Bhlhe40 | 0.001 | 0.06 | 0.05 | 0.14 | 0.001 | 0.93 |
Analysis of circadian gene expression in the gastrocnemius of control and alcohol-treated mice. One-way ANOVAs were used to evaluate changes in gene expression overtime representative of circadian rhythmicity. A sum-of-squares F test was used to determine whether a single cosinor model fit both control and EtOH-treated groups to further indicate differences in rhythmicity between the two groups. Values are P values from control and alcohol-treated female mice at the time points indicated, with bold values indicating significance.