Table 6.
Biochemical analysis of components of the pyruvate dehydrogenase complex
| Control | Cortisol | Control + DCA | Cortisol + DCA | |
|---|---|---|---|---|
| n | 6 | 6 | 5 | 5 |
| PDHE1α | 1.00 ± 0.07 | 1.06 ± 0.05 | 1.07 ± 0.08 | 1.04 ± 0.06 |
| p-S293/PDHE1α | 1.00 ± 0.11 | 0.96 ± 0.17 | 1.01 ± 0.25 | 0.93 ± 0.22 |
| p-S293/PDHE1α | 1.00 ± 0.16 | 0.85 ± 0.14 | 1.01 ± 0.20 | 0.92 ± 0.23 |
| PDK2 | 1.00 ± 0.04 | 0.97 ± 0.08 | 0.86 ± 0.12 | 0.84 ± 0.14 |
| PDK4 | 1.00 ± 0.09 | 1.05 ± 0.05 | 0.92 ± 0.04 | 0.96 ± 0.09 |
| PDH activity | 1.00 ± 0.09 | 1.08 ± 0.04 | 1.17 ± 0.06 | 1.18 ± 0.07 |
Data are expressed as fold-changes in protein expression or PDH activity relative to the control group, means ± SE. DCA, dichloroacetate; PDH, pyruvate dehydrogenase; PDK, pyruvate dehydrogenase kinase; p-S293 PDHE1α, PDHE1α phosphorylated at S293; p-S293/PDHE1α, ratio of phosphorylated PDHE1α to total PDHE1α.