Figure 2.

Glucose consumption of adventitial fibroblasts. The cultured adventitial fibroblasts (AFs) from frozen stock were serum starved for 48 h with LG-DMEM. Conditioned media glucose concentrations were monitored at 24- and 48-h time points with 100 nM AngII (AII) or vehicle (veh: 0.1% H₂O final) treatment in LG-, MG-, or HG-DMEM. Glucose concentration at 24-h (A) and 48-h (B) and glucose consumption rate at 24-h (C) and 48-h (D) were determined (n = 9). Cell numbers were assessed after cells were detached with trypsin (E) (n = 9). Comparison of glucose consumption rates between low and high passages (F) (n = 3). One-way ANOVA with post hoc Tukey’s test showed *P < 0.05, **P < 0.01 and ****P < 0.0001. Data represent means ± SE from independent experiments as indicated. HG, high glucose; LG, low glucose; MG, middle glucose; P, passage.