Figure 4.

Glucose consumption of EC. Conditioned media glucose concentrations were monitored at 24- and 48-h time points with AngII (AII) or vehicle (veh: 0.1% H₂O final) treatment in EC cultured in LG-, MG-, or HG-DMEM. The cultured VSMCs were serum starved for 48 h in LG-DMEM. Glucose concentration at 24 h (A) and 48 h (B) and glucose consumption rate at 48 h (C) were determined. Cell numbers were assessed after cells were detached with trypsin (D). One-way ANOVA with post hoc Tukey’s test showed no statistical significance between the relevant groups. Data represent means ± SE from three independent experiments. AII, angiotensin II; EC, EC, endothelial cell; HG, high glucose; LG, low glucose; MG, middle glucose.