Figure 5.

Simulation on changes in glucose concentrations in vascular cell types. Simulation on changes in glucose concentrations with three types of media (LG 100 mg/dL, MG 275 mg/dL, or HG 450 mg/dL) was made based on the average glucose consumption rates (AF 0.66 mg/day, VSMC 0.25 mg/day, and EC 1.04 mg/day) at confluency on 6-well plates (9.6 cm²). Simulation of 6-well plates with 2 mL of media [AF (A), VSMC (E), and EC (I)]. Simulation of 10 cm dish with 10 mL of AF (B), VSMC (F), and EC (J) with three types of medium. Simulation of 12-well plates with 1 mL of AF (C), VSMC (G), and EC (K) with three types of medium. Simulation of 96-well plates with 0.1 mL of AF (D), VSMC (H), and EC (L) with three types of medium. AF, adventitial fibroblasts; EC, endothelial cell; HG, high glucose; LG, low glucose; MG, middle glucose; VSMC, vascular smooth muscle cell.