Figure 6.

Protein expression in adventitial fibroblasts (AFs) with LG-DMEM starvation and AngII or vehicle stimulation under distinct media glucose conditions for 48 h. The cultured rat aortic AFs from frozen stock were serum starved for 48 h in LG-DMEM. AFs were stimulated with 100 nM AngII (AII) or vehicle (veh) for 48 h as indicated and immunoblotting was performed [ n = 5 (A), n = 4 (B), n = 5 (C), n = 4 (D), n = 5 (E)]. F: signal intensities were used to calculate the expression ratio of each protein to β-actin as indicated. For comparison, protein expression in MG-DMEM without AngII stimulation was set as 1 due to the closest physiological glucose fluctuation. One-way ANOVA with post hoc Tukey’s test showed **P <0.01 and ***P < 0.001. Data represent means ± SE from independent experiments as indicated. AII, angiotensin II; HG, high glucose; LG, low glucose; MG, middle glucose.