Figure 7.

Protein expression in adventitial fibroblasts (Afs) under each medium condition with serum starvation media corresponding to stimulation media. The cultured rat aortic AFs from frozen stock were serum starved for 48 h in LG-, MG-, or HG-DMEM as indicated. AFs were stimulated with 100 nM AngII (AII) or vehicle (veh) in LG-, MG-, or HG-DMEM for 48 h and immunoblotting was performed (A–D). E: signal intensities were used to calculate the expression ratio of each protein to β-actin as indicated. For comparison, protein expression in MG-DMEM without AngII stimulation was set as 1 because it is the closest to physiological glucose levels. One-way ANOVA with post hoc Tukey’s test showed *P < 0.05 and **P < 0.01. Data represent means ± SE from five independent experiments as indicated. AF, adventitial fibroblasts; AII, angiotensin II; HG, high glucose; LG, low glucose; MG, middle glucose.