Figure 9.
Role of AT1 receptor and glycolysis in protein expression in AFs with AngII stimulation. The involvements of AT1 receptor and glycolysis were examined in AngII-induced protein induction in cultured AF from frozen stock. A–G: the cultured rat aortic AFs were serum starved for 48 h in MG-DMEM. The AFs pretreated with vehicle (veh), 10 mM 2DG, or AngII receptor blocker (ARB) 10 μM for 30 min were stimulated with 100 nM Ang II (AII) or veh (H2O) for 48 h. Signal intensities were used to calculate the expression ratio of each protein to β-actin or GAPDH as indicated. For comparison, protein expression in MG-DMEM without AngII stimulation was set as 1 due to the closest physiological glucose fluctuation. One-way ANOVA with post hoc Tukey’s test showed *P < 0.05, **P < 0.01, ***P < 0.001 and ****P <0.0001. Data represent means ± SE from five independent experiments as indicated. AF, adventitial fibroblast; AT1 receptor angiotensin II type-1 receptor; MG, middle glucose.