Figure 2.
Increased activity of lipogenic enzymes. Lipidomic analysis was carried out by thin layer gas chromatography in lipids extracted from plasma collected from adolescent male offspring born to Wt or Hetdb dams (Wt/Wt vs. Wt/Het) that were fed a control diet (CD) or a high-fat/fructose diet (HFFD). The lipogenic enzyme, stearoyl CoA desaturase (SCD1) catalyzes the conversion of saturated fatty acids (SFA), palmitate and stearate, to monounsaturated fatty acids (palmitoleate and oleate) that are incorporated into complex lipids like triglycerides (A). The desaturation index was calculated by the ratio of palmitic acid (B) and stearic acid (C) to their monounsaturated products. The lipogenic index (D), reflecting the rate of de novo lipogenesis, was calculated as the ratio of palmitic acid (16:0) to linoleic acid (18:2n6). The percentage of total saturated fatty acids (SFA) relative to all lipid species (E) was calculated.