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. 2021 Dec 2;601(7894):600–605. doi: 10.1038/s41586-021-04267-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a. Principal component analysis (PCA) plot with PC1 vs PC2 (top) or PC1 vs PC3 (bottom) computed with top 500 variable gene in the bulk transcriptome of individual lineages of blastocyst-like structures (EPI, TE and PrE); stem cell lines: naive and primed hPSCs; hTSCs: blastocyst derived hTSCs (bTS5)²⁰, primed hPSC derived hTSCs (BAP¹⁴ and TM4 protocols⁴⁵; PrE like stem cell lines (RACL or nEND cells²²); naive PSC and TSCs rederived from blastocyst-like structures (see methods). b. Heatmap of key blastocyst and post-implantation lineage markers differentially expressed between TE analogs (TROP2⁺) of the blastocyst-like structures and hTSCs in their bulk transcriptome. c. Pseudotime analysis of human mature TE markers CGB5, CGB7, CGB8 and CGA. Gene expression analysis was performed by using the public data analysis tool (https://bird2cluster.univ-nantes.fr/demo/PseudoTimeUI/). d. Heatmap of key pluripotency related genes differentially expressed between EPI analogs (PDGFR^-/ TROP2^-) in the blastocyst-like structures and primed hPSCs e. Heatmap of key pluripotency related genes or PrE markers differentially expressed between PrE analogs (PDGFRα⁺) in the blastocyst-like structures, naive PSC derived PrE-like cells and nEND cells.