Table 2.
Quantification of process efficiency using enriched TCM/TSCM cells as starting material in P4–P6
| Run | Starting Cell No. | Process Duration | CD4/CD8 Ratio in HARV | Expansion Ratea | T Cell No. in HARV | Transduction Rate of CD45+CD3+ in HARV | CAR T Cell No. in HARV | VCN |
|---|---|---|---|---|---|---|---|---|
| P4 | 0.12 × 108 | 11 days | 73.2%/26.0% | 166× | 1.49 × 109 | 62.6% | 0.93 × 109 | 3.0 |
| P5 | 0.15 × 108 | 12 days | 85.9%/9.6% | 706× | 2.20 × 109 | 41.1% | 0.90 × 109 | 2.4 |
| P6 | 0.14 × 108 | 12 days | 85.9%/9.6% | 305× | 2.28 × 109 | 58.6% | 1.34 × 109 | ND |
n.d., not done.
The expansion rate was calculated from day 2 on.