Table 3.
Physical and medical examinations conducted and collected biosamples at baseline (CARLA-0), first (CARLA-1), second (CARLA-2) and third (CARLA-3) follow-up examination
| Instrument | Time-points | |||
|---|---|---|---|---|
| CARLA-0 (N = 1779) | CARLA-1 (N = 1436) | CARLA-2 (N = 1140) | CARLA-3 (N = ongoing) | |
| Anthropometry | ||||
| Body weight and body height (standing) [15, 72] | x | x | x | x |
| Waist and hip circumference | x | x | x | x |
| Body impedance analysis (BIA) [72] | – | – | – | x |
| 3D Body Scanner [72] | – | – | – | x |
| Cardiovascular system | ||||
| Blood pressure [15, 28] | x | x | x | x |
| Echocardiography, systolic and diastolic function | x | x | – | x |
| 12-leads Electrocardiogram (20-min and 10-s) [30, 31] | x | x | – | xa |
| Ankle–brachial index (ABI) [36)] | x | x | – | x |
| Pulse wave analysis (PWA) | xb | xb | ||
| AGE reader (skin autofluorescence) | – | x (N = 368) | – | x |
| Cognitive function | ||||
| Mini Mental State Examination | – | xc (N = 1020) | – | x |
| Semantic memory, episodic memory, working memory [35, 36] | – | – | – | x |
| Numerical reasoning (fluid intelligence), passive vocabulary (crystallized intelligence) [35] | – | – | – | x |
| Attention/executive, motor coordination [35] | – | – | – | x |
| Gait and balance assessment | ||||
| Timed up and Go Test (TMUG) [39] | – | – | – | x |
| Gait analysis using portable analysis system (Rehagait system) [40] | – | – | – | x |
| Balance assessment using pressure distribution measurement system (Zebris FM System) [41] | – | – | – | x |
| Sensory organ | ||||
| Olfactory test (Sniffin’ sticks 12) [38] | – | – | – | x |
| Bio-specimens | ||||
| Serum | x | x | – | x |
| EDTA whole blood | x | x | – | – |
| EDTA plasma | x | x | – | x |
| EDTA cellular components | x | x | – | x |
| MPA-stabilized EDTA plasma | x | x | – | – |
| Heparin plasma | – | x | – | x |
| Citrate plasma | x | x | – | x |
| Peripheral blood mononuclear cells | – | – | – | x |
| Urine | – | x | – | x |
| Stabilized stool | – | – | – | x |
| Physical activity, physical fitness | ||||
| Hand grip strength [42] | – | – | – | x |
| 7-Day accelerometry [43] | – | – | – | x |
a20-min ECG was not performed. Instead, a 5-min ECG was performed
bSphygmoCor device (CARLA-1) Vascular Explorer (CARLA-3)
cOnly in participants > = 60yrs