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. 2022 Jan 26;13(1):84. doi: 10.1038/s41419-022-04540-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Expression level of NR2F1-AS1 and NR2F1 in the four subtypes (MSS/TP53−, MSS/TP53+, MSI and EMT) of GC in GSE62254 cohort. b, c Gene expression correlation analysis confirmed that NR2F1-AS1 and NR2F1 were related to EMT signaling in GC. d The changes in the GC cell morphology after TGF-beta treatment. e, f The expression of NR2F1 and NR2F1-AS1 were determined by qRT-PCR after TGF-beta treatment. g The stable overexpression of OVOL2 was verified in GC cell lines. h RNA-seq analysis was conducted in GC cells overexpressing OVOL2. i–k RNA-seq and qRT-PCR analysis showed that the expression of NR2F1 and NR2F1-AS1 were greatly decreased after overexpression of OVOL2. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05.