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. 2021 Apr 19;43(2):367–375. doi: 10.1038/s41401-021-00666-9

Fig. 6. AMPK mediates the antifibrotic effect of ORM in vitro.

Fig. 6

Relative mRNA levels of a–c collagen (Col1a1, Col3a1, and Col6a3), d, e ECM regulators (Mmp-13 and Timp-1) and f the collagen fiber cross-linking marker Lox in 3T3-L1 cells treated with 5 ng/mL TGF-β1 for 24 h in the absence or presence of 10 μg/mL ORM and/or dorsomorphin (1 μmol/mL), as indicated (n = 4 per group). g Representative immunofluorescence images of the cells treated in f. h Quantification of LOX fluorescence shown in g (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA with the Tukey’s test.