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. 2021 Sep 22;43(2):354–366. doi: 10.1038/s41401-021-00766-6

Fig. 7. LM8 attenuates renal dysfunction fibrosis in db/db mice.

Fig. 7

Animal experiments were performed as described in “Methods” section. a Kidney to body weight ratios of mice were recorded. Levels of b serum creatine, c blood urea nitrogen (BUN), and d urine albumin to creatinine ratio were examined using commercial kits. e Representative images for hematoxylin and eosin (H&E; upper), Sirius Red (middle), and Masson’s Trichrome (lower) staining of kidney tissues from mice (scale bar = 20 µm). f, g The mRNA levels of Col-4 and TGF-β in the kidney tissues as determined by real-time qPCR. β-Actin was used for normalization. h Protein levels of Col-4 and TGF-β in kidney tissues of mice were determined by Western blot assay. GAPDH was used as loading control. Densitometric quantification is shown in the lower panel. db/m Control db/m group, db/db db/db T2DM mice, LM8-5 5 mg/kg LM8 treatment, LM8-10 10 mg/kg LM8 treatment; mean ± SEM; n = 7 per group; *P < 0.05 compared to db/m; #P < 0.05 compared to db/db.