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. 2021 Sep 30;43(2):285–294. doi: 10.1038/s41401-021-00778-2

Fig. 2. HACE1 regulated neuroinflammation through the Rac1-NADPH oxidase pathway.

Fig. 2

a Levels of NADPH oxidase subunits were determined using Western blot assay. b IP images of HACE1 and Rac1 expression in BV2 cells. c Rac1 translocation was determined using Western blot assay. d Immunofluorescence and statistical analysis of Rac1 in BV2 cells. e The G-LISA assay was applied to measure Rac1 activity. f The Rac1 pull-down assay was used to assess Rac1 activity. The results were presented as mean ± SD, n = 3. The scale bar represents 25 μm. *P < 0.05, **P < 0.01, ***P < 0.001.