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. 2021 Sep 30;43(2):285–294. doi: 10.1038/s41401-021-00778-2

Fig. 3. HACE1 promoted the ubiquitination and degradation of Rac1- GTP.

Fig. 3

a HACE1 siRNA treated BV2 cells were exposed to CHX (10 μg/mL) for the indicated times and analyzed by Western blot assay. b HACE1 siRNA treated BV2 cells were exposed to CHX (8 h), and the cytoplasm and plasma membranes were extracted and analyzed by Western blot assay. c BV2 cells were transfected with HACE1 siRNA and separated on 12% SDS-PAGE and detected by Western blot assay. d 293T cells were transfected with HACE1-cDNA or empty vector, and Rac1 expression in the cytoplasm and plasma membrane were detected by Western blot. e 293T cells were treated with CHX for the indicated times and analyzed by Western blot assay. f 293T cells transfected with HACE1-cDNA were treated with CHX for 8 h, and Rac1 in the cytoplasm and plasma membrane were detected with an anti-Rac1 antibody by Western blot. g 293T cells transfected with HACE1-cDNA, and analyzed by Western blot assay. Results were expressed as mean ± SD, n = 3. *P < 0.05, **P < 0.01.