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. 2021 Apr 8;43(2):417–428. doi: 10.1038/s41401-021-00646-z

Fig. 2. MB reduces TBHP-induced metalloproteinase expression and oxidative stress in hCHs and hFLSs.

Fig. 2

a, i The viability of human OA chondrocytes (hCHs) and human OA fibroblast-like synoviocytes (hFLSs) after TBHP treatment and treatment with or without MB. b, j Representative Western blot results for MMP1, MMP3, MMP13 in hCHs and hFLSs after TBHP treatment and treatment with or without MB. Quantification of the Western blot data from b (c) and j (k). Immunohistochemical staining for MMP1, MMP3, and MMP13 in the affected joint cartilage (d) and synovium (l) of rats (scale bar: 100 μm). Fluorescence images of the DCFH-DA probe for hydrogen peroxide in hCHs (e) and hFLSs (m) in each group (scale bar: 100 μm). f, n Quantitative real-time PCR analysis of IL-1β, IL-6, NOX4, SOD1, and SOD2 mRNA levels in hCHs and hFLSs. g Representative Western blot results for SOX9 and Col II in hCHs after TBHP treatment and treatment with or without MB. h Quantification of the Western blot data from h. All data represent the mean ± S.D. (n = 5). *P < 0.05, **P < 0.01, and ***P < 0.001.