Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 26;13:525. doi: 10.1038/s41467-022-28126-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — A Total bsh (EC:3.5.1.24) gene abundance in WT and CD19^−/− mice is shown. Two-tailed unpaired Student’s t-test; *=p = 0.04. B Correlation between bsh gene abundance and ileal BA concentrations is shown. Two-tailed one-way ANOVA, Fstat(1,20) = 10.66, p = 0.004. C Total 7α-dehydratase (EC:4.2.1.106) gene copies in WT and CD19^−/− mice are shown. Two-tailed unpaired Student’s t-test; ns = p = 0.0651. D Correlation between 7α-dehydratase gene abundance and ileal BA concentrations is shown. Two-tailed one-way ANOVA, Fstat_(1,20) = 0.13, p = ns. E Correlation between Lactobacillaceae abundance and bsh gene abundance is shown. Two-tailed one-way ANOVA, Fstat_(1,23) = 33.48, p < 0.0001. F Heatmaps summarizing descriptive statistics (Pearson r-coefficient and r-squared) of relationship between ileal bacterial bsh gene abundance and specific BA species are shown. Data representative of two replicate experiments. G Correlation between TCA-d4 and CA-d4 abundance (μM) in fecal protein extracts (left panel) and ileal content protein extracts (right panel) from WT and CD19^−/− mice. Two-tailed one-way ANOVA (WT fecal protein extracts), Fstat(1,13) = 18.71_, p = 0.0008. Two-tailed one-way ANOVA (CD19^−/− fecal protein extracts), Fstat_(1,13) = 11.04_, p = 0.006. Two-tailed one-way ANOVA (WT ileal protein extracts), Fstat_(1,7) = 9.186_, p = 0.02. Two-tailed one-way ANOVA (CD19^−/− ileal protein extracts), Fstat(1,8) = 0.0006_, p = 0.98. H Total abundance of deconjugated CA-d4 in fecal and ileal content protein extracts from WT and CD19^−/− mice. Two-tailed unpaired Student’s t-test; ns = non-significant =p = 0.63, ***=p = 0.0002. I Total bsh gene copies in feces and ileal contents of CD19^−/− mice administered B cells from CD19^−/− or WT mice. Two-tailed unpaired Student’s t-test; ns = non-significant =p = 0.68, *=p = 0.04. J Correlation between SI enteropathy and bsh gene copies in feces or ileal contents of CD19^−/− mice administered B cells from CD19^−/− or WT mice. Two-tailed one-way ANOVA (feces), Fstat_(1,13) = 0.13_, p = ns. Two-tailed one-way ANOVA (ileal contents), Fstat_(1,10) = 7.61_, p = 0.01. A–E Data representative of three replicate experiments. G, H Data representative of two replicate experiments. I, J Data representative of two replicate experiments.