Fig. 2. C0818 inhibited DNA synthesis and induced cell cycle arrest in HCC cells.

a Cell proliferation was determined by EdU incorporation assay using flow cytometry. b The cell cycle distribution percentages were determined by flow cytometry. c The expression of cell cycle-regulated proteins was measured by Western blotting. The data are presented as the mean ± SD (n = 3). ^**P < 0.01 and ^***P < 0.001 versus the control.