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. 2021 Apr 6;43(2):446–456. doi: 10.1038/s41401-021-00642-3

Fig. 3. C0818 induces apoptosis in HCC cells.

Fig. 3

a The expression levels of apoptosis-related proteins were measured by Western blotting. b The expression levels of cleaved caspase-3 and cleaved PARP in cells pretreated with z-VAD-fmk were measured by Western blotting. c Quantification of viability (MTT) in cells pretreated with z-VAD-fmk. d Flow cytometric quantification of apoptosis (annexin V/PI staining) in cells pretreated with z-VAD-fmk. e The expression levels of Bax and Bcl-2 were measured by Western blotting. f The mitochondrial membrane potential was measured by flow cytometry. The data are presented as the mean ± SD (n = 3). **P < 0.01 and ***P < 0.001 versus the control, ##P < 0.01 and ###P < 0.001 versus C0818 treatment.