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. 2021 Apr 6;43(2):446–456. doi: 10.1038/s41401-021-00642-3

Fig. 6. C0818 affected the molecular chaperone functions of Hsp90 in HCC cells.

Fig. 6

a C-Raf was analyzed by Western blotting in cells treated with cycloheximide in the absence or presence of C0818. b, c The expression levels of C-Raf and AKT proteins were measured by Western blotting in the cells treated with MG132 or NH4Cl. d C-Raf and AKT protein levels were analyzed by Western blotting in cells transfected with Hsp90 siRNA in the absence or presence of C0818. e Immunoprecipitation was used to evaluate the interaction of Hsp90 with its client proteins C-Raf and AKT. f C-Raf and AKT mRNA levels in HepG2 cells were measured by quantitative real-time PCR; GAPDH was used as an internal control. The results from three independent experiments are shown.