KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CD46 clone TRA-2-10 | Biolegend | Cat #352405 |
| CD55 clone JS11 | Biolegend | Cat #311311 |
| CD71 (TFRC) clone CYIG4 | Biolegend | Cat #334105 |
| Bacterial and virus strains | ||
| NEB Stable Cells | New England Biolabs | Cat #C3040I |
| E. coli NiCo21(DE3) | New England Biolabs | Cat #C2529 |
| Biological samples | ||
| Buffy coats (leukocytes) | New York Blood Center | https://nybloodcenter.org/ |
| Chemicals, peptides, and recombinant proteins | ||
| Lymphoprep | Stem Cell technologies | Cat #07811 |
| Recombinant human IL-2 | Stem Cell Technologies | Cat #78081 |
| ImmunoCult Human CD3/CD28 T Cell Activator | Stem Cell Technologies | Cat #10991 |
| Kanamycin | Sigma-Aldrich | Cat #K1377 |
| IPTG | Sigma-Aldrich | Cat #I6758 |
| Doxycycline hydrochloride | Sigma-Aldrich | Cat #D3447 |
| LIVE/DEAD Fixable Violet Dead Cell Stain | ThermoFisher Scientific | Cat #L34963 |
| Nuclease Buffer 10X | GenScript | Cat #SC1841 |
| 2X RNA Gel Loading Dye | Thermo Fisher Scientific | Cat #R0641 |
| Novex 10% TBE-Urea gels | Thermo Fisher Scientific | Cat #EC68752BOX |
| Critical commercial assays | ||
| EasySep Human CD8 Positive Selection Kit | Stem Cell technologies | Cat #17853 |
| EasySep Human CD4 T cell Isolation Kit | Stem Cell technologies | Cat #17952 |
| Bradford assay | Bio-Rad | Cat #5000201 |
| Direct-zol RNA MicroPrep | Zymo Research | Cat #R2061 |
| SF Cell Line Nucleofector Solution | Lonza | Cat #V4XC-2032 |
| P3 Primary Cell Nucleofector Solution | Lonza | Cat #V4XP-3032 |
| RevertAid RT Reverse Transcription Kit | Thermo Fisher Scientific | Cat #K1691 |
| HiScribe T7 High Yield RNA Synthesis Kit | New England Biolabs | Cat #E2040S |
| HiScribe T7 ARCA mRNA Kit | New England Biolabs | Cat #E2060S |
| Monarch RNA Cleanup Kit | New England Biolabs | Cat #T2050S |
| Experimental models: Cell lines | ||
| HEK293FT | Thermo Fisher Scientific | Cat#R70007 |
| HEK293FT-RfxCas13d-NLS | This paper | N/A |
| Experimental models: Organisms/strains | ||
| Oligonucleotides | ||
| Please refer to Table S2 and S3 | ||
| Recombinant DNA | ||
| pLenti-TRS-Leader-SARS-CoV-2-EGFP | This paper | Addgene 171585 |
| pET28-His-MBP-NLS-RfxCas13d-NLS-HA | This paper | Addgene 171586 |
| pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His | This paper | Addgene 171587 |
| pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast | Wessels et al., 2020 | Addgene 138149 |
| pMD2.G | Trono Lab packaging and envelope plasmids | Addgene 12259 |
| psPAX2 | Trono Lab packaging and envelope plasmids | Addgene 12260 |
| Software and algorithms | ||
| GraphPad Prism 8 | GraphPad | https://www.graphpad.com/ |
| FlowJo 10 | Becton, Dickinson and Company | https://www.flowjo.com/solutions/flowjo |
| Cas13 design tool | Guo X. et. al. bioRxiv (2020) | https://cas13design.nygenome.org/ |
| Other | ||
| HiTrapDEAEFF Sepharose columns | Cytiva | Cat #17505401 |
| HisTrap HP columns | Cytiva | Cat #17524701 |
| HiTrap HeparinHP columns | Cytiva | Cat #17040703 |