FIGURE 5.

Quality control of the spatial transcriptome. (A) Image of Bin 50. Stereo‐seq chip surface arranges the spot patterned array chip. Chips have spots with 200 nm diameter and a centre‐to‐centre distance of 715 nm. Each DNB with a diameter of approximately 220 nm occupies a single spot. Probe on DNB captures mRNA from 10 μm thickness tissue sections covering the chip. The matrix region composed of 50 DNB spacing was named Bin50 for subsequent analysis. (B–G) Influencing factors of RNA capture efficiency, including tissue location (B), sampling method (C), transportation and storage (D), delivery hours of samples (E), sample processing method (F) and storage days at –80°C (G), RIN (H), HE scores (I), RIN < 6, 6–7 or > 7 (J). The Bin50‐UMI/saturability/sequencing total reads represent the RNA capture efficiency on basis of the amount of Bin50 capture, saturation and sequencing