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. 2022 Jan 27;13(1):86. doi: 10.1038/s41419-022-04535-z

Fig. 2. AAV-LAV-BPIFB4 routes the reduction of senescence in aged-aorta and NAD+ plasma levels increase.

Fig. 2

A Freshly isolated aortas from mice were stained for Saβ-gal. On the left, representative photographs (n = 2 young, n = 2 Old-GFP, and n = 2 Old-AAV-LAV-BPIFB4 mice) of Saβ-gal-stained aortas are shown. On the right, the relative densitometric analysis (n = 5 young mice; n = 3 Old-GFP mice; n = 4 Old-AAV-LAV-BPIFB4 mice) shows the percentage of positive areas. Individual values are expressed as mean ± SD. Pairwise comparisons statistically significant are indicated (**P < 0.01). Statistical evaluation was carried out by ordinary one-way ANOVA corrected for Dunnett’s multiple comparisons test (GraphPad® Prism). B Bar graph shows the plasmatic levels of total NAD + in n = 5 young mice, n = 3 Old-GFP mice, and n = 3 Old-AAV-LAV-BPIFB4 mice. Results from each treatment group are expressed as mean ± SD. Pairwise comparisons statistically significant are indicated (*P < 0.05). Statistical evaluation was carried out by ordinary one-way ANOVA and uncorrected Fisher’s LSD test for post-hoc analysis (GraphPad® Prism).