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. 2022 Jan 27;13(1):86. doi: 10.1038/s41419-022-04535-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Blood and bone marrow monocytes in n = 3 Old-GFP mice and n = 3 Old-AAV-LAV-BPIFB4 mice were interrogated by flow cytometry gating on CD45⁺F4/80-Ly6C⁺CD38⁺ cells. Panels show A circulating monocytes and B bone marrow resident monocytes frequency in n = 3 Old GFP mice and n = 3 Old-AAV-LAV-BPIFB4 mice. C Histogram shows the amounts of CD45⁺F4/80-Ly6C⁺CD38⁺ monocytes in bone marrow regarding the stratification in Ly6C^high and Ly6C^low monocytes (*P < 0.05). Results are expressed as mean ± SD. Pairwise comparisons statistically significant are indicated (ANOVA; *P < 0.05, **P < 0.01). Statistical evaluation was carried out by ordinary one-way ANOVA corrected for Tukey’s multiple comparisons test (GraphPad® Prism). D IL-27 and E TNF-α secretion levels in bone marrow microenvironment of Old-GFP mice vs Old-AAV-LAV-BPIFB4 mice (*P < 0.05) expressed as mean ± SD. Statistical evaluation was carried out by ordinary one-way ANOVA corrected for Tukey’s multiple comparisons test (GraphPad® Prism).